Table 1.
Genes interrupted by transposon insertion in colony morphology mutants.
| Mutant(s) | Gene | Locus tag | Gene product | Biological process |
|---|---|---|---|---|
| M1 (nt. 717) | barA | E1N14_00465 | Two‐component sensor histidine kinase BarA | Transcription, Transcription regulation, Two‐component regulatory system |
| M2 (nt. 1057) | nadB | E1N14_01455 | L‐aspartate oxidase | Pyridine nucleotide biosynthesis |
| M3 (nt. 662) | thyA | E1N14_01485 | Thymidylate synthase | Nucleotide biosynthesis, Translation regulation |
|
M4 (nt. 503) M5 (nt. 513) M6 (nt. 177) M7 (nt. 77) M8 (nt. 91) M9 (nt. 119) M10 (nt. 77) M11 (nt. 544) |
E1N14_01730 | E1N14_01730 | Hypothetical protein | – |
| M12 (nt. 208) | nqrA | E1N14_01800 | Na+‐translocating NADH‐quinone reductase subunit A | Ion transport, Sodium transport, Transport |
| M13 (nt. 190) | fusA | E1N14_03520 | Elongation factor G | Protein biosynthesis |
| M14 (nt. 171) | E1N14_03880 | E1N14_03880 | Hypothetical protein | ‐ |
| M15 (nt. 499) | rho | E1N14_05040 | Transcription termination factor rho | Transcription, Transcription regulation, Transcription termination |
|
M16 (nt. 978) M17 (nt. 396) |
gspF | E1N14_05760 | T2SS protein GspF | Protein transport, Transport |
| M18 (nt. 570) | gspD | E1N14_05770 | T2SS protein GspD | Protein transport, Transport |
| M19 (nt. 217) | menF | E1N14_07510 | Isochorismate synthase | Menaquinone biosynthesis |
| M20 (nt. 1574) | menD | E1N14_08030 | 2‐Succinyl‐5‐enolpyruvyl‐6‐hydroxy‐3‐cyclohexene‐1‐carboxylase synthase | Menaquinone biosynthesis |
| M21 a | E1N14_08925 | E1N14_08925 | Type I secretion C‐terminal target domain‐containing protein | Cell communication |
| M22 (nt. 68) | E1N14_09155 | E1N14_09155 | Hypothetical protein | ‐ |
| M23 (nt. 1915) | priA | E1N14_09830 | Primosomal protein N’ | DNA replication |
| M24 (nt. 143) | degS | E1N14_10330 | Outer membrane‐stress sensor serine endopeptidase DegS | Stress response |
| M25 (nt. 61) | E1N14_10530 | E1N14_10530 | L‐sorbosone dehydrogenase | ‐ |
| M26 (nt. 79) | crp | E1N14_10795 | cAMP‐activated global transcriptional regulator CRP | Transcription, Transcription regulation |
| M27 (nt. 239) | mdh | E1N14_12485 | Malate dehydrogenase | Tricarboxylic acid cycle |
| M28 (nt. 902) | E1N14_14620 | E1N14_14620 | LPS O‐antigen length regulator | Lipopolysaccharide biosynthetic process |
| M29 (nt. 567) | E1N14_14660 | E1N14_14660 | Glycosyltransferase family 1 protein | Glycogen biosynthesis |
|
M30 (nt. 963) M31 (nt. 430) |
E1N14_14665 | E1N14_14665 | Delta‐aminolevulinic acid dehydratase | Heme biosynthesis, Porphyrin biosynthesis |
|
M32 (nt. 8) M33 (nt. 137) |
wecG | E1N14_14675 | WecB/TagA/CpsF family glycosyltransferase | Enterobacterial common antigen biosynthetic process |
|
M34 (nt. 566) M35 (nt. 297) M36 (nt. 297) M37 (nt. 90) |
E1N14_14870 | E1N14_14870 | Hypothetical protein | ‐ |
|
M38 (nt. 2499) M39 (nt. 3654) |
purL | E1N14_15175 | Phosphoribosylformylglycinamidine synthase | Purine biosynthesis |
|
M40 (nt. 19) M41 (nt. 191) |
E1N14_17155 | E1N14_17155 | DNA adenine methylase | DNA restriction‐modification system |
| M42 (nt. 618) | recB | E1N14_17705 | Exodeoxyribonuclease V subunit beta | DNA damage, DNA repair |
| M43 (nt. 1494) | edd | E1N14_19200 | Phosphogluconate dehydratase | Carbohydrate metabolism, Gluconate utilization |
Gene abbreviations, locus tag in the S. algae CECT 5071 chromosome and gene annotations are indicated. Biological processes associated with gene products are shown for annotated genes.
a
The open reading frame appears to be truncated (edge of a contig), therefore the transposon insertion site is ambiguous.