Table 5.

Compositional data, intake values, reference points and margin of exposure (MOE) for the individual volatile components of lemon oil expressed classified according to assessment groups. Intake calculations for the individual components are based on the use level of 40 mg/kg in feed for chickens for fattening, the species with the highest ratio of feed intake/body weight. The MOE for each component is calculated as the ratio of the reference point (NOAEL) to the intake. The combined margin of exposure (MOET) is calculated for each assessment group as the reciprocal sum of the reciprocals of the MOE of the individual substances

Essential oil composition			Exposure		Hazard characterisation		Risk characterisation
Assessment group	FLAVIS no	Max conc. in the oil	Max Feed conc.	Daily Intakea	Cramer class	NOAELb	MOE	MOET
Constituent	–	%	mg/kg	mg/kg bw/day	–	mg/kg bw/day	–	–
CG 31, II (Acyclic alkanes)
Myrcene	01.008	2.14	0.856	0.0768	I	44	573
cis‐β‐Ocimene	01.064	0.12	0.050	0.0045	I	44	9,882
trans‐β‐Ocimene	01.018	0.11	0.043	0.0039	I	44	11,346
MOET CG 31, II				0.0852				517
CG 31, III (Cyclohexene hydrocarbons)
Limonene	01.045	66.7	26.68	2.3951	I	250	104
γ‐Terpinene	01.020	11.5	4.584	0.4115	I	250	608
β‐Bisabolene	01.028	0.87	0.346	0.0311	I	250	8039
Terpinolene	01.005	0.55	0.222	0.0199	I	250	11,139
α‐Terpinene	01.019	0.30	0.121	0.0108	I	250	23,053
α‐Phellandrene	01.006	0.26	0.102	0.0092	I	250	27,302
MOET CG 31, III				2.8777				87
CG 31, IVe (Benzene hydrocarbons, alkyl)
p‐Cymene	01.002	0.59	0.236	0.0212	I	154	7,257
CG 31, V (Bi‐, tricyclic, non‐aromatic hydrocarbons)
α‐Pinene	01.004	16.8	6.720	0.6033	I	222	368
β‐Pinene	01.003	3.63	1.452	0.1304	I	222	1,703
Sabinene	01.059	2.65	1.060	0.0952	I	222	2,333
α‐Thujene	n.a.	0.56	0.223	0.0200	I	222	11,099
β‐Caryophyllene	01.007	0.33	0.130	0.0117	I	222	18,964
(E)‐α‐bergamotene	n.a.	0.57	0.228	0.0204	I	3	147
Bicyclogermacrene	n.a.	0.03	0.012	0.0011	I	3	2,785
MOET CG 31, V				0.8820				91
CG 31, VI (macrocyclic non‐aromatic hydrocarbons)
δ‐Germacrene	01.042	0.01	0.004	0.0003	I	3	9,283
CG 1
Decanal	05.010	0.19	0.0768	0.0069	I	120	17,405
Octanal	05.009	0.14	0.0568	0.0051	I	120	23,534
Octan‐1‐ol	02.006	0.11	0.0456	0.0041	I	120	29,314
MOET CG 1				0.0161				7,459
CG 3
Geranial	05.188	2.08	0.832	0.0747	I	345	4,619
Neral	05.170	1.43	0.572	0.0514	I	345	6,719
Neryl acetate	09.213	0.68	0.272	0.0244	I	345	14,150
Geranyl acetate	09.011	0.51	0.203	0.0182	I	345	18,913
MOET CG 3				0.1687				2,045
CG 4
Citronellol	02.011	0.13	0.051	0.0046	I	50	10,878
Citronellal	05.021	0.07	0.028	0.0025	I	50	20,180
Citronellyl acetate	09.012	0.07	0.026	0.0023	I	50	21,422
MOET CG 4				0.0094				5,315
CG 6
Linalool	02.013	0.45	0.179	0.0161	I	117	7,273
α‐Terpineol	02.014	0.16	0.066	0.0059	I	250	42,451
MOET CG 6				0.0220				6,209
CG 8
l‐Fenchone	n.a.	0.17	0.068	0.0061	II	0.9	148
Bornyl acetate	09.017	0.01	0.005	0.0008	I	3	6,962
Trans‐Carveol	02.062	0.01	0.004	0.0004	I	3	7,595
cis‐p‐2,8‐Menthadien‐1‐ol	n.a.	0.01	0.002	0.0004	III	0.15	696
Cis‐Carveol	02.062	0.01	0.002	0.0002	I	3	13,924
MOET CG 8				0.0073				117
CG 32 (epoxides)
cis‐Limonene oxide	n.a.	0.07	0.026	0.0023	I	3	1,285
trans‐Limonene oxide	n.a.	0.03	0.013	0.0012	I	3	2,532
MOET CG 32								852
α‐Thujone	n.a.	0.006	0.002	0.0002	III	8	37,131

^aIntake calculations for the individual components are based on the use level of 5 mg/kg in feed for chickens for fattening, the species with the highest ratio of feed intake/body weight. The MOE for each component is calculated as the ratio of the reference point (NOAEL) to the intake. The combined margin of exposure (MOET) is calculated for each assessment group as the reciprocal of the sum of the reciprocals of the MOE of the individual substances.

^bValues in bold refer to those components for which the NOAEL value was available, values in italics are the 5th percentile of the distribution of NOAELs of the corresponding Cramer Class, other values (plain text) are NOAELs extrapolated by using read‐across.