Table 12.

Compositional data, intake values, reference points and margin of exposure (MOE) for the individual volatile components of the residual fraction of expressed lemon oil classified according to assessment groups. Intake calculations for the individual components are based on the use level of 12 mg/kg in feed for chickens for fattening, the species with the highest ratio of feed intake/body weight. The MOE for each component is calculated as the ratio of the reference point (NOAEL) to the intake. The combined margin of exposure (MOET) is calculated for each assessment group as the reciprocal sum of the reciprocals of the MOE of the individual substances

Essential oil composition			Exposure		Hazard characterisation		Risk characterisation
Assessment group	FLAVIS no	Max conc. in the oil	Max feed conc.	Daily intakea	Cramer class	NOAELb	MOE	MOET
Constituent	–	%	mg/kg	mg/kg bw per day	–	mg/kg bw per day	–	–
CG 31 II (Acyclic alkanes)
Myrcene	01.008	0.56	0.069	0.0062	I	44	7,091
trans‐Ocimene	01.018	0.22	0.027	0.0024	I	44	18,234
cis‐Ocimene	01.064	0.11	0.013	0.0012	I	44	36,796
MOET CG 31, II				0.0098				4,483
CG 31, III (Cyclohexene hydrocarbons)
Limonene	01.045	56.8	6.816	0.6119	I	250	409
γ‐Terpinene	01.020	23.3	2.796	0.2510	I	250	996
β‐Bisabolene	01.028	2.71	0.325	0.0292	I	250	8,563
Terpinolene	01.005	0.86	0.104	0.0093	I	250	26,891
MOET CG 31, III				0.9014				277
CG 31, IVe (Benzene hydrocarbons, alkyl)
p‐Cymene	01.002	0.58	0.070	0.0063	I	154	24,605
CG 31, V (Bi‐, tricyclic, non‐aromatic hydrocarbons)
β‐Pinene	01.003	3.38	0.406	0.0364	I	222	6,097
(E)‐α‐bergamotene	n.a.	2.05	0.246	0.0221	I	3	136
β‐Caryophyllene	01.007	1.28	0.154	0.0138	I	222	16,100
Bicyclogermacrene	n.a.	0.91	0.023	0.0021	I	3	1,458
Sabinene	01.059	0.45	0.053	0.0048	I	222	46,309
α‐trans β‐bergamotene	n.a.	0.10	0.012	0.0011	I	3	2,678
MOET CG 31, V				0.0803				115
CG 31, VI (macrocyclic non‐aromatic hydrocarbons)
Germacra‐1(10),4(14),5‐triene	n.a.	0.006	0.001	0.0001	I	3	46,414
CG 3
Geranial	05.188	11.2	1.344	0.1207	I	345	2,859
Neral	05.170	8.94	1.073	0.0963	I	345	3,582
Neryl acetate	09.213	2.32	0.278	0.0250	I	345	13,804
Geranyl acetate	09.011	1.57	0.188	0.0169	I	345	20,398
MOET CG 3				0.2589				1,333
CG 4
Citronellol	02.011	0.55	0.066	0.0059	I	50	8,424
Citronellyl acetate	09.012	0.34	0.041	0.0037	I	50	13,571
Citronellal	05.021	0.12	0.015	0.0013	I	50	37,735
MOET CG 4				0.0109				4,568
CG 6
α‐Terpineol	02.014	0.48	0.058	0.0052	I	250	48,247
Linalool	02.013	0.46	0.055	0.0049	I	117	23,817
MOET CG 6				0.0115				14,601
CG 8
l‐Fenchone	n.a.	0.17	0.020	0.0018	II	0.91	518
Spathulenol	n.a.	0.09	0.011	0.0010	III	0.15	148
Trans‐Carveol	02.062	0.06	0.007	0.0006	I	3	5,063
Cis‐Carveol	02.062	0.03	0.004	0.0004	I	3	8,191
Pinocarveol		0.03	0.004	0.0004	I	3	7,957
cis‐p‐2,8‐Menthadien‐1‐ol	n.a.	0.03	0.003	0.0003	III	0.15	557
MOET CG 8				0.0044				92
CG 32 (epoxides)
cis‐Limonene oxide	n.a.	0.13	0.015	0.0014	I	3	2,210
trans‐Limonene oxide	n.a.	0.09	0.010	0.0009	I	3	3,201
MOET CG 32								1,307
α‐Thujone	n.a.	0.016	0.002	0.0002	III	8	46,414

^aIntake calculations for the individual components are based on the use level of 12 mg/kg in feed for chickens for fattening, the species with the highest ratio of feed intake/body weight. The MOE for each component is calculated as the ratio of the reference point (NOAEL) to the intake. The combined margin of exposure (MOET) is calculated for each assessment group as the reciprocal of the sum of the reciprocals of the MOE of the individual substances.

^bValues in bold refer to those components for which the NOAEL value was available, values in italics are the 5th percentile of the distribution of NOAELs of the corresponding Cramer Class, other values (plain text) are NOAELs extrapolated by using read‐across.