Table 22.

Compositional data, intake values, reference points and margin of exposure (MOE) for the individual components of lime essential oil classified according to assessment groups. Intake calculations for the individual components are based on the use level of 8.5 mg/kg in feed for chickens for fattening, the species with the highest ratio of feed intake/body weight. The MOE for each component is calculated as the ratio of the reference point (NOAEL) to the intake. The combined margin of exposure (MOET) is calculated for each assessment group as the reciprocal sum of the reciprocals of the MOE of the individual substances

Essential oil composition			Exposure		Hazard characterisation		Risk characterisation
Assessment group	FLAVIS no	Max conc. in the oil	Max feed conc.	Daily intakea	Cramer class	NOAELb	MOE	MOET
Constituent	–	%	mg/kg	mg/kg bw/day	–	mg/kg bw/day	–	–
CG 31 II (Acyclic alkanes)
Myrcene	01.008	1.34	0.114	0.0102	I	44	4,303
α‐Farnesene	01.040	0.89	0.076	0.0068	I	44	6,472
cis‐β‐Ocimene	01.064	0.23	0.020	0.0018	I	44	24,748
β‐Farnesene	01.041	0.14	0.012	0.0011	I	44	40,043
MOET CG 31, II				0.0199				2,221
CG 31, III (Cyclohexene hydrocarbons)
Limonene	01.045	38.1	3.239	0.2907	I	250	860
γ‐Terpinene	01.020	16.9	1.437	0.1290	I	250	1,939
Terpinolene	01.005	11.2	0.952	0.0855	I	250	2,925
α‐Terpinene	01.019	3.79	0.322	0.0289	I	250	8,644
β‐Bisabolene	01.028	2.44	0.207	0.0186	I	250	13,427
α‐Phellandrene	01.006	0.67	0.057	0.0051	I	250	48,899
1‐Methyl‐1,4‐cyclohexadiene	n.a.	0.10	0.009	0.0008	I	3	3,854
β‐Elemene	n.a.	0.05	0.004	0.0004	I	3	7,561
3‐Methylene‐1,5,5‐trimethylcyclohexene	n.a.	0.05	0.004	0.0004	I	3	8,547
1‐Methyl‐1,4‐cyclohexadiene	01.077	0.02	0.002	0.0002	I	3	17,093
(3R‐trans)‐3‐methyl‐6‐(1‐methylvinyl)cyclohexene	n.a.	0.02	0.002	0.0002	I	3	17,093
MOET CG 31, III				0.5597				350
CG 31, IVe (Benzene hydrocarbons, alkyl)
p‐Cymene	01.002	3.07	0.261	0.0234	I	154	5,862
CG 31, V (Bi‐, tricyclic, non‐aromatic hydrocarbons)
β‐Pinene	01.003	2.94	0.250	0.0224	I	222	9,896
α‐Pinene	01.004	1.63	0.139	0.0124	I	222	17,849
Camphene	01.009	0.62	0.052	0.0047	I	222	47,152
β‐Caryophyllene	01.007	0.48	0.041	0.0037	I	222	60,110
(E)‐ α‐bergamotene	n.a.	0.79	0.067	0.0060	I	3	499
β‐Santalene	n.a.	0.12	0.010	0.0009	I	3	3,360
cis‐ α‐Bergamotene	n.a.	0.11	0.010	0.0009	I	3	3,510
MOET CG 31, V				0.0510				359
CG 31, VI (macrocyclic non‐aromatic hydrocarbons)
3,7,10‐Humulatriene	01.043	0.14	0.011	0.0010	I	3	2,912
Germacrene B	n.a.	0.06	0.005	0.0005	I	3	6,552
MOET CG 31, VI				0.0086				2,016
CG 6
α‐Terpineol	02.014	10.50	0.893	0.0801	I	250	3,120
α‐Terpineol	n.a.	1.69	0.144	0.0129	I	250	19,386
1‐Terpinenol	02.096	0.89	0.075	0.0068	I	250	37,020
cis‐β‐Terpineol	n.a.	0.96	0.081	0.0073	I	250	34,235
4‐Terpineol	02.072	0.67	0.057	0.0051	I	250	48,826
MOET CG 6				0.1131				2,228
CG 8
Fenchyl alcohol	02.038	0.71	0.060	0.0054	I	3	555
Borneol	02.016	0.69	0.059	0.0053	I	3	566
Isoborneol	02.059	0.07	0.006	0.0005	I	15	27,302
MOET CG 8				0.0112				555
CG 16
1,4‐Cineole	03.007	2.36	0.201	0.0180	II	100	5,553
1,8‐Cineole	03.001	1.96	0.167	0.0150	II	100	6,686
MOET CG 16				0.0330				3,034

^aIntake calculations for the individual components are based on the use level of 8.5 mg/kg in feed for chickens for fattening, the species with the highest ratio of feed intake/body weight. The MOE for each component is calculated as the ratio of the reference point (NOAEL) to the intake. The combined margin of exposure (MOET) is calculated for each assessment group as the reciprocal of the sum of the reciprocals of the MOE of the individual substances.

^bValues in bold refer to those components for which the NOAEL value was available, values in italics are the 5th percentile of the distribution of NOAELs of the corresponding Cramer Class, other values (plain text) are NOAELs extrapolated by using read‐across.