Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 28;14(3):1073–1083. doi: 10.1111/1751-7915.13758

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 1 — Expression and mitochondria targeting of nitrogenase proteins in S. cerevisiae. Immunoblot analysis of yAnfD, yAnfK, yAnfH and yAnfG proteins in total protein extracts (TE), in isolated mitochondria (Mito) and in ‘post‐mitochondrial supernatant’ fractions (PM) from S. cerevisiae strains GF15 (A), GF17 (B), GF16 (C) and GF18 (D). Total extracts and mitochondria isolation were prepared from aerated cultures of galactose‐induced cells. Antibodies recognizing A. vinelandii AnfD, AnfK, AnfH and AnfG proteins, HSP60 (mitochondrial marker protein), or tubulin (cytosolic marker) were used for immunoblotting analysis.