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. 2021 Apr 1;24(4):102387. doi: 10.1016/j.isci.2021.102387

Figure 2.

Figure 2

Examples of immunostaining and preserving GFP fluorescence with tissue clearing on the developing mouse heart

(A and B) Immunohistochemistry combined with BABB on ED 9.5 mouse embryo, smooth muscle actin antibody (SMA) in red labeling the myocardium, CD31 (PECAM-1) in green staining the endocardium (B), and DAPI nuclear staining (not very distinct at this low magnification) in blue.

(C and D) Preservation of natural GFP fluorescence with CUBIC tissue clearing on ED 10.5 (C) and ED 14.5 (D) in mouse Connexin 40 - GFP (Cx40-GFP) embryo hearts with superimposed autofluorescence in red. Ventricular trabeculae and atria are positive for Cx40 at these stages. Autofluorescent blood is present in the ventricles (C). All images were captured with confocal microscopy. Scale bar represents 100 μm in all figures.