Coronary vasculature visualization in the developing hearts
(A) Vasculature was injected with DiI in a quail ED9 embryo.
(B) Coronary arteries were visualized on heart surface of an ED18.5 Cx40-GFP mouse embryo cleared with CUBIC. Imaged on a confocal microscope.
(C) Analysis of the DiI injected heart with coronary vessels pseudocolored to indicate depth within the ventricular wall, ED13 quail embryo.
(D) Juvenile mouse heart with coronary vasculature injected with Microfil and main coronary arteries indicated with color: Right coronary artery (orange) and its branch, septal artery (red), and left coronary artery (yellow). Imaged on a micro-CT scanner.