Table 2.

Heart and heart vasculature tissue clearing methods

	Method name	Key components	Clearing time	Protocol modification for heart or heart vasculature
Solvent based	BABB	Benzoic Acid/Benzyl Benzoate	Days	Miller et al. (2005); Kolesova et al. (2016); Ivins et al., 2016
	FluorClear BABB	Tert-butanol/BABB	Hours	Epah et al. (2018)
	DBE	DiBenzylEther	Hours	Kolesova at al.,2016
	3DISCO	Dichlormethane/DBE	Hours-Days	Belle et al. (2017); Epah et al. (2018)
	iDISCO	Dichlormethane/DBE	Hours-Days	Belle et al. (2017); Epah et al. (2018), Goodyer et al. (2019)
	Ethanol-Eci	Ethyl-3-phenylprop-2-enoate	Days	Klingberg et al. (2017)
	FASTClear	THF/DBE	45 min	Perbellini et al. (2017)
Simple immersion	FRUIT	Fructose/Triglycerol/Urea	Days	Xu et al. (2019a), 2019b
	MACS	MXDA/Sorbitol	Days	Xu et al. (2019a), 2019b; Zhu et al. (2020)
	SeeDB	Fructose/Thioglycerol	Week	Tainaka et al. (2014)
	Sucrose	Sucrose	1 day	Tsai et al., (2009)
Hyperhydratation	CUBIC	4M Urea/50% Sucrose	Days	Kolesova et al. (2016); Nehrhoff et al. (2016); Tainaka et al. (2014)
	SCALE S	4M Urea/Sorbitol	Days-weeks	Li et al. (2016); Matryba et al. (2019)
	SCALE A2	4M Urea/10% Glycerol	Weeks	Kolesova et al. (2016)
	SUT	Urea/SDS	Days	Wang et al. (2018); Matryba et al. (2019)
Hydrogel embedding	ACT-PRESTO	Hydrogel/SDS	Days	Kim et al. (2016)
	Clarity	FocusClear/80% Glycerol	Days	Kolesova et al. (2016)
	SCM	Hydrogel	Weeks	Sung et al. (2016); Matryba et al. (2019)
	PACT	Hydrogel/SDS	Days-Weeks	Treweek et al. (2015), Xu et al. (2019a), 2019b
	PARS	Hydrogel/SDS	Days	Treweek et al. (2015)
	SWITCH	SDS/Thioglycerol	Weeks	Murray et al. (2015)