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. 2021 May;64(5):579–591. doi: 10.1165/rcmb.2020-0312OC

Figure 2.

Figure 2.

RIPK3 deficiency leads to worse outcome during S. pne infection. Mice were intranasally instilled with S. pne (ATCC 6303) or PBS. (A) Survival study showed that Ripk3−/− mice were more susceptible to S. pne infection than WT mice (n = 10 for each group). *P < 0.05 by Mantel Cox test. (B) Pulmonary bacterial burden of WT or Ripk3−/− mice at indicated times after infection (n = 5 for each group). (C and D) Total cell counts and protein quantification in BAL fluid of WT or Ripk3−/− mice at Day 3 after infection (n = 3 PBS; n = 5 S. pne). *P < 0.05 and ***P < 0.001 by one-way or two-way ANOVA test. Data are presented as mean ± SEM. (E) At 3 days after infection, mouse lungs were harvested for histology. Representative photomicrographs (hematoxylin and eosin stained) taken from each group were shown. Scale bars, 20 μm. (F) Survival study of Ripk3fl/fl; LysM-Cre−/− or Ripk3fl/fl; LysM-Cre+/− mice (n = 10 for each group). *P < 0.05 by Mantel Cox test. (G) Survival study of Ripk3fl/fl; Spc-Cre−/− or Ripk3fl/fl; Spc-Cre+/− mice (n = 10 for each group). Technical and biological replicates for infection experiments, N = 10. Experiments were repeated at least three times, with representative findings shown. NS = no significant difference.