Skip to main content
. Author manuscript; available in PMC: 2021 Jun 2.
Published in final edited form as: Nature. 2020 Dec 2;588(7839):688–692. doi: 10.1038/s41586-020-2996-z

Extended Data Fig. 7. Stress granules are not induced by GAG.

Extended Data Fig. 7.

a, Immunofluorescence staining of G3BP1 (green), DDX3X (red) and bone marrow-derived macrophage (BMDM) nuclei (blue) in unprimed BMDMs 40 min after transfection with GAG or incubation with arsenite (Ars). Representative images (n ≥ 2 independent experiments). b, Quantification of the percentage of stress granule-positive cells after transfection with GAG, vehicle (DOTAP) alone or Ars. (n > 10 biologically independent fields of cells). Data are mean +/− SEM. c, Immunofluorescence staining of G3BP1 (green), DDX3X (red) and BMDM nuclei (blue) in unprimed BMDMs 15 h after infection with A. fumigatus. Representative images (n ≥ 2 independent experiments). d,e, Immunoblot analysis of pro–caspase-1 (pro-Casp1; p45) and the active caspase-1 subunit (p20) of BMDMs assessed 3 h after transfection with vehicle (DOTAP), (d) GAG or (e) d-GAG. Representative images (n ≥ 2 independent experiments). f, Immunoblot analysis of caspase-1 from BMDMs left untreated (medium alone [Med]) or infected with A. fumigatus (A.f) WT or deletion mutant Δgt4c (multiplicity of infection [MOI], 10). Representative images (n ≥ 2 independent experiments). (a, c) Scale bars, 10 μm.