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. Author manuscript; available in PMC: 2021 Jun 2.
Published in final edited form as: Nature. 2020 Dec 2;588(7839):688–692. doi: 10.1038/s41586-020-2996-z

Extended Data Fig. 4. Over-synthesis of GAG induces hyper-inflammasome activation.

Extended Data Fig. 4.

a, Immunofluorescence staining of A. fumigatus (A.f) GAG (green) and bone marrow-derived macrophage (BMDM) nuclei (blue) in unprimed BMDMs 4 h after infection with A. fumigatus (A. f) wild type (WT) or Δugm1 resting conidia (multiplicity of infection [MOI], 10). Scale bars, 10 μm. Representative images (n ≥ 3 independent experiments). b, Immunoblot analysis of pro–caspase-1 (pro-Casp1; p45) and the active caspase-1 subunit (p20) of unprimed BMDMs left untreated (medium alone [Med]) or assessed 20 h after infection with the indicated live A. f resting conidia genotype (WT or A. f deletion mutant Δugm1) (MOI, 10). Representative images (n ≥ 3 independent experiments). c, Release of IL-1β from unprimed BMDMs left uninfected (Med) or assessed 20 h after infection with A. f (MOI, 10). **P = 0.0046 (unpaired two-tailed t-test). (n = 4 biologically independent samples). Data are mean +/− SEM.