Fig. 4.

ALG3 promotes cancer stem-like traits of breast cancer cells. a Four different GSEA gene sets showed that ALG3 expression was positively correlated with CSC-associated gene signatures in a published breast cancer dataset. b NANOG, OCT4, and SOX2 expression were up-regulated in ALG3-tansduced cells and down-regulated in ALG3-knocked out cells. α-tubulin was detected as a loading control in the Western blot. c Sphere formation capacity was shown in four breast cancer cell lines. d Representative and statistic graph of flow cytometry analysis of MCF-7, ZR-75-30, MDA-MB-231, and SUM159PT cell lines stained with antibodies against cell surface markers CD44 and CD24. Data were analyzed by Student’s t-test and one-way ANOVA with Tukey’s multiple comparison test. Each bar represents the mean ± SD of three independent experiments. e Representative and statistic graph of flow cytometry detecting the percentage of side population cells of each sample. Data were analyzed by Student’s t-test and one-way ANOVA with Tukey’s multiple comparison test. Each bar represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01