Figure 3.

Normal physiological response of macrophage-specific Adrb2 knockout mice to fasting and refeeding. (A) Weights of Adrb2^fl/fl (n = 20) and Adrb2^fl/flLyz2^Cre/+ (n = 17) chow-fed 3-month-old male mice after 16-hour fast. (B) Blood glucose, (C) serum insulin and (D) serum free fatty acids in the same mice after 16-hour fast and after refeeding for 2 and 6 h. (E) Suppression of lipolysis in response to refeeding, calculated by dividing the refed FFA values from the fasted value for each mouse. (F) Weights of indicated tissues isolated from Adrb2^fl/fl (n = 8) and Adrb2^fl/flLyz2^Cre/+ (n = 8) chow-fed 4-month-old male mice after 16-hour fast. (G) Flow cytometry quantification of eWAT macrophage number and (H) the expression of indicated surface proteins in the macrophage population, normalized to the average of Adrb2^fl/fl group. (I) qPCR expression analysis of indicated genes in the CD11b-positive eWAT cell fraction. All graphs show means ± SEM. In (B–D), p values of 2-way ANOVA are indicated on the graph. In (I), ∗ indicates p < 0.05 compared between groups using a two-tailed Student's t-test.