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. 2021 Mar 25;48:101220. doi: 10.1016/j.molmet.2021.101220

Figure 4.

Figure 4

Normal physiological response of macrophage-specific Adrb2 knockout mice to cold exposure. (A) Ucp1 and (B) Elovl3 mRNA in BAT, (C) Ucp1 and (D) Itgam mRNA in scWAT and (E) serum TNFα and (F) IL-10 levels in Adrb2fl/fl (n = 25 male, 4 female) and Adrb2fl/flLyz2Cre/+ (n = 18 male, 4 female) chow-fed 3-month-old mice, exposed to 4 °C for times varying between 0 and 5.5 h. (G) Body weights, (H) rectal temperature, (I) tissue weights and qPCR expression analysis of indicated genes in (J) BAT and (K) scWAT of Adrb2fl/fl (n = 11) and Adrb2fl/flLyz2Cre/+ (n = 8) chow-fed 3-month-old male mice, exposed to 4 °C for 72 h. All graphs show means ± SEM. In (A-B, D-F), p values of linear regression and ANCOVA analyses are indicated on the graph. In (G–H), p values of two-way ANOVA are indicated on the graph.