Table 2.

Overview of the hepatic differentiation timeline, the final concentration of growth factors and small molecules used

Day	Cell type	Final concentration of growth factors and small molecules
D-2	hPSCs	N/A
D-1		N/A
D0	hPSCs	Activin A 100 ng/mL + CHIR99021 3 μM + LY294002 10 μM
D1		N/A
D2		N/A
D3	Definitive Endoderm	Activin A 50 ng/mL
D4		N/A
D5	Anterior Definitive Endoderm	Activin A 50 ng/mL
D6		BMP4 20 ng/mL + FGF10 10 ng/mL
D7		BMP4 20 ng/mL + FGF10 10 ng/mL
D8		N/A
D9	Hepatoblasts	BMP4 20 ng/mL + FGF10 10 ng/mL
D10		OSM 30 ng/mL + HGF 50 ng/mL
D11		N/A
D12		OSM 30 ng/mL + HGF 50 ng/mL
D13		N/A
D14		OSM 30 ng/mL + HGF 50 ng/mL
D15		N/A
D16		OSM 30 ng/mL + HGF 50 ng/mL
D17		N/A
D18		OSM 30 ng/mL + HGF 50 ng/mL
D19		N/A
D20		OSM 30 ng/mL + HGF 50 ng/mL
D21		N/A
D22		OSM 30 ng/mL + HGF 50 ng/mL
D23		N/A
D24	Hepatocyte-like cells	OSM 30 ng/mL + HGF 50 ng/mL