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Components and quantity of DNA mix
| Component | Quantity per well | Quantity per triplicate wells (3×) |
|---|---|---|
| Luciferase reporter plasmid (such as pGL4.10 vector containing the promoter of interest) | 490 ng | 1.47 μg |
| Overexpression plasmid (such as pCDH vector containing the protein-coding sequence of interest) | 500 ng | 1.5 μg |
| pRL-TK Renilla Vector (internal control reporter vector) | 10 ng | 30 ng |
| Total | 1 μg | 3 μg |
