RPMI/2% B27 differentiation medium
| Reagent | Final concentration | Amount |
|---|---|---|
| RPMI 1640 | N/A | 485 mL |
| B-27 Supplement (50×), Minus Vitamin A | 2% | 10 mL |
| MEM-NEAA | 1% | 5 mL of stock |
| Total | N/A | 500 mL |
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