Figure 2.

Flow cytometry analysis of the negative control, normoxic, and hypoxic cells

HepG2 cells were cultured in normoxic (Middle) and hypoxic (right) incubator for 3 days. And then the normoxic and hypoxic cells were digested, washed with pre-heated buffer and hypoxia-pretreated medium, respectively. They were suspended by each medium at a concentration of 5 μM MitoSOX and incubated for 20 min, while part of normoxic HepG2 cells was separated to be treated with DMSO for 20 min (left, Negative control). Post washing, all the cells were performed with flow cytometry analysis immediately.