REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Critical commercial assays | ||
MitoSOX | Thermo Fisher | Cat #M36008 |
Trypsin-EDTA (0.25%) | Thermo Fisher | Cat #25200056 |
Opti-MEMTM | Thermo Fisher | Cat #31985070 |
LipofectamineTM 3000 | Thermo Fisher | Cat #L3000015 |
Hanks’s balanced salt solution (with Ca2+ & Mg2+) | Beyotime | Cat #C0219 |
1 × PBS buffer | Sangon Biotech | Cat #B540626 |
HepG2 | ATCC cell lines | Cat #HB-8065 |
DMEM basic (1×) | Gibco | Cat #C11995500BT |
DMSO | Sigma | Cat #41639-100ML |
Software and algorithms | ||
FlowJoTM 10 | BD Biosciences | https://www.flowjo.com |
GraphPad Prism | GraphPad | https://www.graphpad.com |
Other | ||
Countstar® Bio Tech system | Countstar | https://aberinstruments.com/ |
BD LSRFortessa™ Flow Cytometer | BD Biosciences | https://www.bdbiosciences.com/ |
IV5100FL inverted fluorescence microscope | Olympus Imaging | https://olympus-imaging.cn |
Tri-gas CO2 incubator (150i) | Thermo Fisher | https://www.thermofisher.com/ |