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Generation and confirmation of TH-TdTomato knock-in hPSC lines (optional step)
(A) Schematic overview of the CRISPR-mediated gene targeting approach.
(B) Bright-field image of a WA01 colony after targeting. Scale bar: 500 μm.
(C) 5′, 3′, and 5′3′ PCRs confirming TH-TdTomato vector integration into selected WA01 and BJ-SiPS hPSC clones.
(D) Sequencing results validating correct vector integration for two clones using the “TH-TdTomato Forward” primer for the 5′ PCR product.
