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Seeding of monolayer hPSCs into 3D spinner flasks
(A) Brightfield image of a healthy WA01 TH monolayer hPSC colony prior to seeding into flasks. Scale bar: 100 μm.
(B) Brightfield image of WA01 TH hPSCs at 70%–80% confluency prior to seeding into flasks. Scale bar: 500 μm.
(C) Image of spinner flasks on a nine-position stir plate in the incubator.
