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. 2021 Apr 19;2(2):100463. doi: 10.1016/j.xpro.2021.100463

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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QC immunostaining of young midbrain organoids for neuronal and DN markers

(A and B) IF staining of WA01 TH midbrain neurons for TH (green) and RFP (red) in (a) cryosection of a D20 organoid; (b) D21 dissociated neurons. The nuclei are counterstained with Hoechst (blue).

(C and D) IF staining of WA01 TH midbrain neurons for TUBB3 (green) and RFP (red) in (c) cryosection of a D20 organoid; (d) D21 dissociated neurons. The nuclei are counterstained with Hoechst (blue).

(E) Quantification of D21 TH⁺ cells as a percentage of total cells in B and similar images (n=50 per replicate). Error bars represent SEM.

(F) Quantification of D21 TH⁺ cells (as denoted by RFP staining for the TH-TdTomato reporter) as a percentage of TUBB3⁺ cells in D and similar images (n=35 per replicate). Error bars represent SEM.

All scale bars: 100 μm.