REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit Monoclonal Anti-FOXA2 (Clone EPR4466)	Abcam	Cat#Ab108422; RRID: AB_11157157
Rabbit Polyclonal Anti-GAPDH	Abcam	Cat#Ab9485; RRID: AB_307275
Mouse Monoclonal Anti-GFAP (Clone GA5)	Millipore	Cat#MAB360; RRID: AB_11212597
Rabbit Polyclonal Anti-RFP	Rockland	Cat#600-401-379; RRID: AB_2209751
Mouse Monoclonal Anti-TH (Clone LNC1)	Millipore	Cat#MAB318; RRID: AB_2201528
Mouse Monoclonal Anti-Beta III Tubulin or TUBB3 (Clone 2G10)	Abcam	Cat#Ab78078; RRID: AB_2256751
Chemicals, peptides, and recombinant proteins
Accutase® Cell Detachment Solution	Innovative Cell Technologies	Cat#AT104; RRID: AB_2869384
B-27™ Supplement (50×), Minus Vitamin A	Gibco	Cat#12587010; RRID: N/A
Bovine Albumin Fraction V (7.5% Solution)	Gibco	Cat#15260037; RRID: N/A
CHIR99021	Tocris	Cat#4423; RRID: N/A
DAPT	Cayman Chemical	Cat#13197; RRID: N/A
DB-cAMP/Dibutyryl-cAMP	Biolog	Cat#D 009; RRID: N/A
DirectPCR Lysis Reagent (Cell)	Viagen Biotech	Cat#302-C; RRID: N/A
DMEM/F-12, GlutaMAX™ Supplement	Gibco	Cat#10565018; RRID: N/A
DMEM, High Glucose	Gibco	Cat#11965092; RRID: N/A
DNase Vial (D2)	Worthington Biochemical Corporation	Cat#LK003170; RRID: N/A
Geltrex™ LDEV-Free, hESC-Qualified, Reduced Growth Factor Basement Membrane Matrix	Gibco	Cat#A1413302; RRID: N/A
HBSS with 10 mM HEPES, Without Phenol Red	STEMCELL Technologies	Cat#37150; RRID: N/A
Hibernate A	BrainBits	Cat#HA; RRID: N/A
Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water	Invitrogen	Cat#H3570; RRID: N/A
Laminin Mouse Protein, Natural	Gibco	Cat#23017015; RRID: N/A
L-Ascorbic Acid (White Crystalline Powder)	Fisher Scientific	Cat#BP351-500; RRID: N/A
LDN193189	Stemgent	Cat#04-0074; RRID: N/A
Leibovitz's L-15 Medium	Gibco	Cat#11415064; RRID: N/A
N-2 Supplement (100×)	Gibco	Cat#17502048; RRID: N/A
Opti-MEM™ I Reduced Serum Medium	Gibco	Cat#31985062; RRID: N/A
PBS, pH 7.4 (-CaCl2, -MgCl2)	Gibco	Cat#10010023; RRID: N/A
PDS Kit, Papain Vial	Worthington Biochemical Corporation	Cat#LK003176; RRID: N/A
Penicillin-Streptomycin (10,000 U/mL)	Gibco	Cat#15140122; RRID: N/A
Poly-L-Ornithine Hydrobromide	Sigma-Aldrich	Cat#P3655; RRID: N/A
Proteinase K Solution (20 mg/mL)	Viagen Biotech	Cat#501-PK; RRID: N/A
Purmorphamine	STEMCELL Technologies	Cat#72204; RRID: N/A
Puromycin Dihydrochloride	Gibco	Cat#A1113803; RRID: N/A
Recombinant Human BDNF Protein	R&D Systems	Cat#248-BD; RRID: N/A
Recombinant Human GDNF Protein	R&D Systems	Cat#212-GD; RRID: N/A
SAG	Cayman Chemical	Cat#11914; RRID: N/A
SB431542	Stemgent	Cat#04-0010-10; RRID: N/A
StemFlex™ Medium	Gibco	Cat#A3349401; RRID: N/A
Thiazovivin	Selleck Chemicals	Cat#S1459; RRID: N/A
Trypan Blue Solution, 0.4%	Gibco	Cat#15250061; RRID: N/A
Y-27632 Dihydrochloride	Tocris	Cat#1254; RRID: N/A
Critical commercial assays
Fontana-Masson Stain Kit (Melanin Stain)	Abcam	Cat#Ab150669; RRID: N/A
Lipofectamine™ Stem Transfection Reagent	Invitrogen	Cat#STEM00003; RRID: N/A
P3 Primary Cell 4D-NucleofectorTM X Kit S	Lonza	Cat#V4XP-3032; RRID: N/A
Experimental models: Cell lines
Human: BJ SiPS-D induced pluripotent stem cells	Harvard University	Cat#BJ SiPS-D: RRID: CVCL_X741
Human: WA01 (H1) embryonic stem cells	WiCell Research Institute	Cat#WA01; RRID: CVCL_9771
Oligonucleotides
Primer: TH-TdTomato Forward: AGCCCTCTAGCCTCATCCTC	This paper	RRID: N/A
Primer: TH-TdTomato Reverse: GAGCCTCTGGAGCTGCTTG	This paper	RRID: N/A
Primer: 5Prime Reverse: ACATCCCCTGCTTGTTTCAA	This paper	RRID: N/A
Primer: 3Prime post-Cre Forward: TCCCTCAGACCCTTTTAGTCA	This paper	RRID: N/A
Primer: ACTB Forward: CCTGGCACCCAGCACAAT	Pilegaard et al., 2003	RRID: N/A
Primer: ACTB Reverse: GCCGATCCACACGGAGTACT	Pilegaard et al., 2003	RRID: N/A
Primer: FOXA2 Forward: GGGGTAGTGCATCACCTGTT	Jo et al., 2016	RRID: N/A
Primer: FOXA2 Reverse: CCGTTCTCCATCAACAACCT	Jo et al., 2016	RRID: N/A
Primer: GIRK2 (KCNJ6) Forward: TGCCCAAAGAGGAACTGGAAAT	This paper	RRID: N/A
Primer: GIRK2 (KCNJ6) Reverse: AGTCAACTTCGTAGAACCCGTC	This paper	RRID: N/A
Primer: LMX1A Forward: GCAAAGGGGACTATGAGAAGGA	PrimerBank	Cat#291327512c1; RRID: N/A
Primer: LMX1A Reverse: CGTTTGGGGCGCTTATGGT	PrimerBank	Cat#291327512c1; RRID: N/A
Primer: NURR1 (NR4A2) Forward: CAGGCGTTTTCGAGGAAAT	Kirkeby et al., 2017	RRID: N/A
Primer: NURR1 (NR4A2) Reverse: GAGACGCGGAGAACTCCTAA	Kirkeby et al., 2017	RRID: N/A
Recombinant DNA
Plasmid: pTAHR TH-p2a-TD:Tomato (floxed selection Puro)	Ahfeldt et al., 2020; Addgene	Cat#135814; RRID: N/A
CRISPR 1: pTACR TH1-p2aGFP	Ahfeldt et al., 2020; Addgene	Cat#135815; RRID: N/A
CRISPR 2: pTACR TH2-p2aGFP	Ahfeldt et al., 2020; Addgene	Cat#135816; RRID: N/A
Plasmid: pCAG-Cre:GFP	Matsuda and Cepko, 2007; Addgene	Cat#13776; RRID: Addgene_13776
Software and algorithms
CellProfiler	https://cellprofiler.org/	RRID:SCR_007358
Fiji	https://fiji.sc/	RRID:SCR_002285
Other
4D-NucleofectorTM Core Unit	Lonza	Cat#AAF-1002B; RRID: N/A
Cell Culture Microplate, 96-well, PS, F-bottom (Chimney Well), μClear®, Black, CELLCOAT®, Poly-L-Lysine, Lid with Condensation Rings, 5 pcs./bag	Greiner	Cat#655936; RRID: N/A
Corning® 125 mL Disposable Spinner Flask with 70 mm Top Cap and 2 Angled Sidearms, Sterile	Corning	Cat#3152; RRID: N/A
Corning® Costar® Ultra-Low Attachment Multiple Well Plate	MilliporeSigma	Cat#CLS3471; RRID: N/A
Countess™ Cell Counting Chamber Slides	Invitrogen	Cat#C10228; RRID: SCR_019815
Countess™ II FL Automated Cell Counter	Invitrogen	Cat#AMQAF1000; RRID: N/A
Dropper Bulb, Natural Latex Rubber, 2 mL, 12 per Case	Research Products International	Cat#142823; RRID: N/A
Falcon® Round-Bottom Tubes with Cell Strainer Cap, 5 mL	STEMCELL Technologies	Cat#100-0087; RRID: N/A
Fisherbrand™ Disposable Borosilicate Glass Pasteur Pipets	Fisher Scientific	Cat#13-678-20D; RRID: N/A
Fisherbrand™ Sterile Cell Strainers (70 μm)	Fisher Scientific	Cat#22-363-548; RRID: N/A
pluriStrainer® 300 μm, 25 pcs. – Sterile (Cell Strainer)	pluriSelect	Cat#43-50300-03; RRID: N/A
pluriStrainer® 500 μm, 25 pcs. – Sterile (Cell Strainer)	pluriSelect	Cat#43-50500-03; RRID: N/A
Stirrers, Magnetic, Nine-position, Dura-Mag	Chemglass Life Sciences	Cat#CLS-4100-09; RRID: N/A
Variable Speed 2D Rocker	USA Scientific	Cat#2527-2000; RRID: N/A