Figure 2.

Structural characterization of S1H peptide.A, ribbon diagram of S1H peptide helix. Residues that make contact with the hGHR are colored green and indicated with arrows. Residues colored blue point away from the interface and are not thought to make contact with the hGHR. Primary sequence of S1H is shown below the ribbon diagram; residues that make contact with the hGHR are underlined. N-terminus is shown capped with an acetyl group (Ac); C terminus is an amide (-NH₂). S1H helix exported from PDB ID: 1HWG. Figure rendered in PyMol. B, wavelength-dependent circular dichroism spectra of S1H peptide (20 μM in solution). Red spectrum shows S1H in PBS, and blue spectrum represents S1H in PBS supplemented with 30% (v/v) TFE. All spectra were collected at 25 °C. hGHR, human growth hormone receptor; PBS, phosphate buffered saline; TFE, 2,2,2-trifluoroethanol.