Table 2.
Femoral microarchitecture and dimensions assessed by microcomputed tomography of 1-month-old BGLAP-Cre;RosaNotch3 male and female mice and sex-matched littermate controls
| Trabecular and cortical bone | Males |
Females |
||
|---|---|---|---|---|
| Control n = 9 | Cre+/−;RosaNotch3 n = 6 | Control n = 12 | Cre+/−;RosaNotch3 n = 7 | |
| Distal femur trabecular bone | ||||
| Bone volume/total volume (%) | 13.0 ± 2.6 | 22.4 ± 3.9b | 9.7 ± 1.6 | 23.5 ± 7.2b |
| Trabecular separation (μm) | 167 ± 15 | 146 ± 17b | 186 ± 18 | 170 ± 61 |
| Trabecular number (1/mm) | 6.0 ± 0.5 | 7.5 ± 1.0b | 5.4 ± 0.5 | 7.3 ± 2.3b |
| Trabecular thickness (μm) | 34 ± 3 | 35 ± 1 | 31 ± 2 | 37 ± 2b |
| Connectivity density (1/mm3) | 369 ± 83 | 852 ± 180b | 297 ± 71 | 942 ± 429b |
| Structure model index | 1.9 ± 0.3 | 0.7 ± 0.3b | 2.2 ± 0.2 | 0.4 ± 0.2b |
| Density of material (mg HA/cm3) | 825 ± 22 | 862 ± 18b | 808 ± 28 | 840 ± 27b |
| Distal femur dimensions | ||||
| Periosteal perimeter (mm) | 6.3 ± 0.2 | 6.4 ± 0.3 | 6.0 ± 0.7 | 6.3 ± 0.2 |
| Anteroposterior length (mm) | 1.8 ± 0.1 | 1.7 ± 0.1 | 1.7 ± 0.1 | 1.7 ± 0.1 |
| Mediolateral width (mm) | 2.7 ± 0.1 | 2.6 ± 0.1 | 2.7 ± 0.1 | 2.5 ± 0.1 |
| Femoral midshaft cortical bone | ||||
| Bone volume/total volume (%) | 98.0 ± 1.3 | 47.5 ± 4.5b | 98.8 ± 0.6 | 53.1 ± 8.2b |
| Porosity (%) | 2.0 ± 1.3 | 52.5 ± 4.5b | 1.2 ± 0.6 | 46.9 ± 8.2b |
| Cortical thickness (μm) | 95 ± 6 | 36 ± 3b | 95 ± 5 | 36 ± 5b |
| Total area (mm2) | 1.6 ± 0.1 | 2.0 ± 0.2b | 1.5 ± 0.1 | 1.8 ± 0.2b |
| Bone area (mm2)a | 0.43 ± 0.03 | 0.37 ± 0.09 | 0.40 ± 0.03 | 0.30 ± 0.06b |
| Periosteal perimeter (mm) | 4.4 ± 0.1 | 5.0 ± 0.3b | 4.3 ± 0.2 | 4.8 ± 0.2b |
| Endocortical perimeter (mm) | 3.7 ± 0.1 | 4.0 ± 0.2b | 3.7 ± 0.1 | 4.0 ± 0.1b |
| Density of material (mg HA/cm3) | 1018 ± 38 | 934 ± 11b | 1011 ± 39 | 916 ± 43b |
| p Moment of inertia (mm4) | 0.183 ± 0.02 | 0.182 ± 0.04 | 0.166 ± 0.025 | 0.157 ± 0.045 |
Microcomputed tomography was performed in distal femurs for trabecular bone and midshaft for cortical bone from 1-month-old BGLAP-Cre;RosaNotch3 mice and sex-matched littermate controls.
a
Segmented bone. Values means ± SD.
b
Significantly different between control and Cre+/−;RosaNotch3, p < 0.05 by unpaired t test.