Table 3.
Femoral microarchitecture and dimensions assessed by microcomputed tomography of 1-month-old Dmp1-Cre;RosaNotch3 male and female mice and sex-matched littermate controls
| Trabecular and cortical bone | Males |
Females |
||
|---|---|---|---|---|
| Control n = 12 | Cre+/−;RosaNotch3 n = 11 | Control n = 4 | Cre+/−;RosaNotch3 n = 7 | |
| Distal femur trabecular bone | ||||
| Bone volume/total volume (%) | 11.3 ± 1.8 | 17.1 ± 3.4b | 9.6 ± 0.9 | 14.4 ± 3.3b |
| Trabecular separation (μm) | 170 ± 5 | 152 ± 13b | 182 ± 13 | 162 ± 33 |
| Trabecular number (1/mm) | 5.9 ± 0.2 | 7.0 ± 0.5b | 5.6 ± 0.3 | 6.6 ± 1.3 |
| Trabecular thickness (μm) | 31 ± 3 | 31 ± 3 | 29 ± 1 | 29 ± 2 |
| Connectivity density (1/mm3) | 384 ± 52 | 962 ± 217b | 395 ± 52 | 908 ± 341b |
| Structure model index | 2.1 ± 0.2 | 1.4 ± 0.5b | 2.1 ± 0.1 | 1.6 ± 0.3b |
| Density of material (mg HA/cm3) | 785 ± 22 | 799 ± 22 | 776 ± 7 | 768 ± 20 |
| Distal femur dimensions | ||||
| Periosteal perimeter (mm) | 6.1 ± 0.2 | 6.1 ± 0.2 | 5.7 ± 0.4 | 6.2 ± 0.1 |
| Anteroposterior length (mm) | 1.8 ± 0.2 | 1.7 ± 0.1 | 1.7 ± 0.1 | 1.7 ± 0.1 |
| Mediolateral width (mm) | 2.5 ± 0.1 | 2.5 ± 0.1 | 2.4 ± 0.1 | 2.4 ± 0.1 |
| Femoral midshaft cortical bone | ||||
| Bone volume/total volume (%) | 96.8 ± 2.4 | 85.3 ± 6.5b | 97.2 ± 1.7 | 81.3 ± 9.0b |
| Porosity (%) | 3.2 ± 2.4 | 14.7 ± 6.5b | 2.8 ± 1.7 | 18.7 ± 9.0b |
| Cortical thickness (μm) | 88 ± 8 | 55 ± 11b | 80 ± 7 | 48 ± 8b |
| Total area (mm2) | 1.4 ± 0.1 | 1.4 ± 0.1 | 1.3 ± 0.1 | 1.4 ± 0.1 |
| Bone area (mm2)+ | 0.38 ± 0.03 | 0.292 ± 0.06b | 0.32 ± 0.02 | 0.26 ± 0.01b |
| Periosteal perimeter (mm) | 4.2 ± 0.1 | 4.2 ± 0.2 | 4.0 ± 0.1 | 4.2 ± 0.1 |
| Endocortical perimeter (mm) | 3.6 ± 0.1 | 3.7 ± 0.1 | 3.4 ± 0.2 | 3.7 ± 0.1b |
| Density of material (mg HA/cm3) | 955 ± 32 | 917 ± 17b | 927 ± 31 | 895 ± 19 |
| p Moment of inertia (mm4) | 0.151 ± 0.02 | 0.120 ± 0.03b | 0.111 ± 0.01 | 0.103 ± 0.01 |
Microcomputed tomography was performed in distal femurs for trabecular bone and midshaft for cortical bone from 1-month-old Dmp1-Cre;RosaNotch3 mice and sex-matched littermate controls.
a
Segmented bone. Values means ± SD.
b
Significantly different between control and Cre+/−;RosaNotch3, p < 0.05 by unpaired t test.