Table 7.

Primers used for qRT-PCR

Gene	Strand	Sequence	GenBank accession number
Alpl	Forward	5'-TGGTATGGGCGTCTCCACAGTAACC-3'	NM_007431
	Reverse	5'-CTTGGAGAGGGCCACAAAGG-3'
Bglap	Forward	5'-GACTCCGGCGCTACCTTGGGTAAG-3'	NM_001037939
	Reverse	5'-CCCAGCACAACTCCTCCCTA-3'
Dkk1	Forward	5'-CCCTCCCTTGCGCTGAAGATGAGGAGT-3'	NM_010051
	Reverse	5'-CGCTTTCGGCAAGCCAGAC-3'
Hes1	Forward	5′-ACCAAAGACGGCCTCTGAGCACAGAAAGT-3′	NM_008235
	Reverse	5′-ATTCTTGCCCTTCGCCTCTT-3′
Hey1	Forward	5′-ATCTCAACAACTACGCATCCCAGC-3′	NM_010423
	Reverse	5′-GTGTGGGTGATGTCCGAAGG-3′
Hey2	Forward	5′-AGCGAGAACAATTACCCTGGGCAC-3′	NM_013904
	Reverse	5′-GGTAGTTGTCGGTGAATTGGACCT-3′
HeyL	Forward	5′-CAGTAGCCTTTCTGAATTGCGAC-3′	NM_013905
	Reverse	5′-AGCTTGGAGGAGCCCTGTTTC-3′
HeyLa	Forward	5'-TCCTCACCCGTCAGA-3′	NM_013905
	Reverse	5'-AGGCACCATGTAACTCA-3′
Notch1	Forward	5'-GTCCCACCCATGACCACTACCCAGTTC-3′	NM_008714
	Reverse	5'-GGGTGTTGTCCACAGGGGA-3′
Notch2	Forward	5'-TGACGTTGATGAGTGTATCTCCAAGCC-3′	NM_010928
	Reverse	5'-GTAGCTGCCCTGAGTGTTGTGG-3′
Notch3	Forward	5′-CCGATTCTCCTGTCGTTGTCTCC-3′	NM_008716
	Reverse	5′-TGAACACAGGGCCTGCTGAC-3′
Notch3NICD	Forward	5′-CATCCTTATTTGACCCCGTC-3′	NM_008716
	Reverse	5′-TGGCATTGGTAGCAGTTG-3′
Notch4	Forward	5′-CCAGCAGACAGACTACGGTGGAC-3′	NM_010929
	Reverse	5′-GCAGCCAGCATCAAAGGTGT-3′
Rpl38	Forward	5′-AGAACAAGGATAATGTGAAGTTCAAGGTTC-3′	NM_001048057; NM_001048058; NM_023372
	Reverse	5′-CTGCTTCAGCTTCTCTGCCTTT-3′
Sost	Forward	5′-AGGAATGATGCCACAGAGGTC-3′	NM_024449
	Reverse	5′-CTGGTTGTTCTCAGGAGGAGGCTC-3′
Tnfrsf11b	Forward	5′-CAGAAAGGAAATGCAACACATGACAAC-3′	NM_008764
	Reverse	5′-GCCTCTTCACACAGGGTGACATC-3′
Tnfsf11	Forward	5′-TATAGAATCCTGAGACTCCATGAAAAC-3′	NM_011613
	Reverse	5′-CCCTGAAAGGCTTGTTTCATCC-3′
Wisp1	Forward	5′-TCCAGGAGTTAAGTGATTTGCTCA-3′	NM_018865
	Reverse	5′-CATGTTACATGACACTGGGCTTC-3′

GenBank accession numbers identify transcript recognized by primer pairs.

^aUsed for experiment shown in Figure 3.