Transcriptional control of leptin-stimulated GH-containing cells. Dispersed pituitary cell cultures from adult control mice (24 hours) treated with transcriptional inhibitors (1 hour) prior to leptin stimulation (3 hour). Statistical analysis by two-way ANOVA followed by Tukey’s post-hoc test. In all cases, the source of variation came only from treatment with leptin, resulting in a higher number of growth hormone (GH) cells: (A) F1,16 = 27.49, P < .0001; (B) F1,20 = 30.16, P < .0001; (C) F1,22 = 14.34, P = .001. Interaction: (A) F1,16 = 75.41, P < .001; (B) F1,20 = 53.32, P < .001; (C) F1,22 = 14, P = .001