RNA luciferase reporter assay and Musashi co-association. A,B, Fluc assays for Tshb mRNA 3’ UTR (A) or Prl mRNA 3’ UTR (B). C, MSI1 immunoprecipitation assay and Prl mRNA enrichment. *Significant differences. Statistical analysis by ANOVA followed by Bonferroni’s post hoc test to detect differences between means. A, F3,6 = 65.51, P < .0001. B, F3,6 = 9.95, P = .012. C, Significance by Student’s t test with Welch’s correction. t = 2.781, df = 3.00, P = .034; n is indicated. NS, not significant