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. 2021 Apr 29;16:36. doi: 10.1186/s13020-021-00444-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Photomicrographs of regions of ovaries showing immunohistochemical expression of Ki-67 in the different treated groups (×200). a Negative control group showing strong immunopositive staining in the granulosa cell layer with weak immunoreactivity in the theca cells and stromal cells. b PCOS group showing strong immunopositive reaction in theca cells and interstitial stromal cells. c Clomiphene treated group showing strong immunopositive staining in the granulosa cell layer. d Actaea racemosa (AR) treated group showing strong immunolabeling in granulosa cell layer and slight immunopositive staining in theca cells and stromal cells. e Vitamin C group showing intense immunolabeling in the theca cells and interstitial stromal cells. f AR combined with vitamin C group showing very strong immunopositive staining in granulosa cell layer with a faint reaction in the theca cells and interstitial stromal cells