Fig. 3.

ZBTB28 induced cell cycle arrest and promoted cell apoptosis. a, b Flow cytometry analysis of cell cycle of CaSki and HeLa cells which was overexpression pcDNA 3.1 or pcDNA-ZBTB28 by PI staining. c EdU incorporation in the growing CaSki cells that with/without pcDNA-ZBTB28 were analyzed. d Transmission electron micrograph of apoptotic cells: the proportion of apoptotic bodies were increased in ZBTB28-transfected CaSki and HeLa cells, relative to the pcDNA 3.1-transfected control cells (CaSki: 4000x, Scale bar: 5 μm; HeLa: 6000x, Scale bar: 2 μm). e Ectopically expressed ZBTB28 induced apoptosis in cervical cancer cells were tested by AO/EB staining assay. f Flow cytometry analysis of apoptotic cell population by Annexin V/PI staining. All statistical data were shown as mean ± SEM. *p < 0. 05, **p < 0. 01, ***p < 0. 001