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. 2021 Apr 25;785:147270. doi: 10.1016/j.scitotenv.2021.147270

Fig. 4.

Fig. 4

The mean recovery rates of the AccuPlex™ SARS-CoV-2 reference material (SeraCare, USA) from wastewater. The virus reference material was concentrated from wastewater samples using (A) PEG6000 precipitation and (B) the adsorption-extraction method. Viral RNA extraction was carried out using the NucleoSpin™ RNA Virus kit and detection by either the Luna® Universal Probe One-Step RT-qPCR Kit or the TaqPath™ 1-Step Multiplex Master Mix as indicated. Values are means of three triplicates and error bars indicate standard deviation. P values for the paired t-test comparing the RT-qPCR master mixes following virus precipitation/concentration and RNA extraction are also presented.