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. 2021 Apr 30;40:148. doi: 10.1186/s13046-021-01943-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — KLF5 transcriptionally activates BBOX1-AS1 in NSCLC cells. a Online JASPAR software was utilized to predict the possible binding sites of trascription factor KLF5 on BBOX1-AS1 promoter region. b GEPIA database displayed the expression profile of KLF5 in LUSC. c The correlation between BBOX1-AS1 and KLF5 expression was analyzed by bioinformatics tool GEPIA. d qRT-PCR assays were performed to determine the effect of KLF5 overexpression or knockdown on BBOX1-AS1 expression in A549 and SK-MES-1 cells. e ChIP assay was used to examine the binding of KLF5 with BBOX1-AS1 promoter at the predicted sites (P1 and P2). f The schematic diagram of luciferase reporter vectors containging wild type or mutant P2 sequence of BBOX1-AS1 promoter. g Dual luciferase reporter assay was conducted in A549 cells transfected with wt-P2 or mut-P2 reporter and vector or pcDNA-KLF5. h Dual luciferase reporter assay was performed in SK-MES-1 cells transfected with wt-P2 or mut-P2 reporter and si-NC or si-KLF5. ***P < 0.001