a, Bulk RNA sequencing analysis of microglia isolated from the frontal cortex of adolescent (P40) mice from WT (n = 4), C4−/− (n = 5), and hC4A/- (n = 5), hC4B/- (n = 4), and hC4A/A (n = 2) groups. Heatmap representation of differentially expressed genes between all experimental groups shows no significant transcriptional profile difference between any two groups. b-c, Normalized gene counts for the TREM2/DAP12 signaling pathway (b) and TAM receptor genes (c) were calculated for WT (n = 4), C4−/− (n = 5), hC4A/- (n = 5), hC4B/- (n = 4), and hC4A/A (n = 2; ns = P > 0.05, Kruskal-Wallis test with Dunn’s test). Bar graphs show mean ± s.e.m.