Table 1. Beam profiles in fluorescent dyes, with and without AO, compared to no-coverslip conditions.
Beam parameter | No coverslip, no AO | Coverslip and AO | Coverslip, no AO | ||||
---|---|---|---|---|---|---|---|
Excitation wl, nm | 488 | 561 | 488 | 561 | 488 | 561 | |
Beam waist a , µm | 1.9 | 1.9 | 3.3 | 3.1 | 5.2 | 5.2 | |
Beam length b , µm | 28.2 | 21.4 | 60.8 | 44.9 | 90.4 | 79.4 |
Measurement errors ±0.1 µm (std).
Beam length = 2x Rayleigh range.