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. 2021 Apr 16;9:651449. doi: 10.3389/fcell.2021.651449

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Copyright © 2021 Gaussmann, Gopalswamy, Eberhardt, Reuter, Zou, Schliebs, Erdmann and Sattler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PEX5 - PEX14 interaction by isothermal titration calorimetry. ITC experiments were performed as triplicates. (A) Titration of 50 μM PEX5 in aqueous buffer into a 30 μM aqueous solution of PEX14. (B) The same experiment in the presence of 44 μM bicelle corresponding to full saturation of PEX5 and PEX14 with 0.9- fold and 1.5- fold molar excess, respectively. (C) The titration experiments in membrane-like environment show reduced binding enthalpies ΔH and entropic contribution –TΔS and slightly reduced affinity compared to experiments in aqueous solution.