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RT-PCR Primer Sequences
| Gene | Sequence (5’−3’) | Annealing Temp (°C) |
|---|---|---|
| ALDH | 60.4 | |
| Forward | GGAAGCCATCCAGTTCATCA | |
| Reverse | GTCTCCGCGATCATCTTCTT | |
| Biglycan | 62.4 | |
| Forward | ACCTCCCTGAGACCCTCAAT | |
| Reverse | TTGTTGTCCAAGTGCAGCTC | |
| Keratocan | 60.4 | |
| Forward | TGACCTGCAGCACAATAAGC | |
| Reverse | TGAGTCTCAGGAAGGCCACT | |
| GAPDH | 62.4 | |
| Forward | GGGTCATCATCTCTGCACCT | |
| Reverse | GGTCATAAGTCCCTCCACGA |
