Figure 1.

Analysis of transcriptome and translatome of human DC infected with Mtb and treated with rapamycin. DC isolated from 4 healthy donors were left unstimulated (CTRL), treated with rapamycin (RAPA) or infected with Mtb alone (MTB) or in presence of rapamycin added 4 h post infection (MTB+RAPA). Cells were harvested 16 h post infection for total and polysome-associated RNA isolation and microarray analysis. (A) Principal component analysis of gene expression profile of samples. Triangles indicate total RNA samples (TOTAL), circles refer to RNA fraction associated with polysome chains (HEAVY). (B) Heatmap showing hierarchical clustering of sample correlation. The dendrogram clusters together the significantly regulated treatment conditions with most similar expression profiles among the 4 donors. Correlation is represented by a color code according to the legend where yellow indicates higher correlation. A color code was used to distinguish among treatment conditions (CTRL, RAPA; MTB, MTB+RAPA), RNA type (TOTAL vs HEAVY) and DC culture prepared from different subjects (D1, D2, D3, D4).