TABLE 5.
Concentrations of iodides (I–) and iodates (IO3–) in roots and leaves of lettuce in hydroponics NFT Experiment No. 1 (speciation of iodine analyzed HPLC-ICP-MS/MS) as well percentage of the iodides and iodates in relation to the total iodine.
| Experiment No. 1. Hydroponic NFT/Part of plant | Treatments | (mg⋅kg–1 D.W.) | Percentage of the sum of iodides and iodates in relation to the total I content* (%) | Percentage of the sum of analyzed organic-I** in relation to the total I content* (%) | Theoretical percentage share of the content of other unanalyzed organic or inorganic I compounds in the total I content (%) | ||
| Iodides (I–) | Iodates (IO3–) | The sum of two forms of I: I– and IO3– | |||||
| Leaves | Control | 2.34 ± 0.005b | 0.0272 ± 0.0008d | 2.365 ± 0.005b | 57.7 ± 0.80c | 3.26 ± 0.11d | 39.0 |
| SA | 0.97 ± 0.006a | 0.0293 ± 0.0009d | 0.998 ± 0.006a | 56.3 ± 0.53c | 2.39 ± 0.26c | 41.4 | |
| KIO3 | 15.06 ± 0.042f | 0.0146 ± 0.0006b | 15.078 ± 0.042f | 73.4 ± 0.64f | 0.71 ± 0.05b | 25.9 | |
| KIO3+SA | 17.49 ± 0.140g | 0.0139 ± 0.0001b | 17.504 ± 0.140g | 56.0 ± 0.33c | 0.16 ± 0.02a | 43.9 | |
| 5-ISA | 97.06 ± 0.189i | 0.0488 ± 0.0006f | 97.113 ± 0.189i | 67.6 ± 1.77e | 0.25 ± 0.01ab | 32.1 | |
| 3,5-diISA | 8.63 ± 0.044d | 0.0002 ± 0.0001a | 8.625 ± 0.044d | 5.3 ± 0.60a | 0.75 ± 0.01b | 93.9 | |
| KIO3+V | 14.12 ± 0.041e | 0.0215 ± 0.0004c | 14.143 ± 0.041e | 63.1 ± 0.36d | 0.30 ± 0.06ab | 36.6 | |
| KIO3+SA+V | 18.92 ± 0.229h | 0.0023 ± 0.0001a | 18.918 ± 0.229h | 84.1 ± 1.20h | 0.66 ± 0.01ab | 15.3 | |
| 5-ISA+V | 118.25 ± 0.623j | 0.0022 ± 0.0001a | 118.252 ± 0.624j | 72.4 ± 0.12f | 0.31 ± 0.01ab | 27.3 | |
| 3,5-diISA+V | 3.46 ± 0.009c | 0.0428 ± 0.0017e | 3.507 ± 0.010c | 23.6 ± 0.20b | 7.01 ± 0.12f | 69.4 | |
| Roots | Control | 7.70 ± 0.0187b | 0.8780 ± 0.0007e | 8.577 ± 0.019b | 22.7 ± 0.32g | 2.41 ± 0.04 | 74.9 |
| SA | 0.16 ± 0.0025a | 0.0137 ± 0.0001a | 0.169 ± 0.002a | 0.3 ± 0.01a | 0.70 ± 0.02 | 99.0 | |
| KIO3 | 10.87 ± 0.0131c | 0.9914 ± 0.0006ef | 11.866 ± 0.013c | 12.8 ± 0.12d | 0.55 ± 0.05 | 86.7 | |
| KIO3+SA | 34.99 ± 0.4050e | 0.2436 ± 0.0037d | 35.235 ± 0.408e | 25.6 ± 0.24h | 0.40 ± 0.04 | 74.0 | |
| 5-ISA | 72.58 ± 0.0764f | 1.7800 ± 0.0428g | 80.364 ± 0.119f | 14.0 ± 0.23e | 0.86 ± 0.03 | 85.1 | |
| 3,5-diISA | 8.12 ± 0.0183b | 0.9589 ± 0.0005f | 9.080 ± 0.018b | 1.1 ± 0.01ab | 9.18 ± 0.10 | 89.8 | |
| KIO3+V | 13.53 ± 0.0426d | 0.0531 ± 0.0004ab | 13.583 ± 0.042d | 16.4 ± 0.15f | 1.32 ± 0.06 | 82.3 | |
| KIO3+SA+V | 11.27 ± 0.0564c | 0.1136 ± 0.0009c | 11.382 ± 0.057c | 12.5 ± 0.23d | 2.10 ± 0.06 | 85.4 | |
| 5-ISA+V | 119.26 ± 1.6836g | 0.9511 ± 0.0266ef | 120.212 ± 1.710g | 6.2 ± 0.08c | 0.44 ± 0.00 | 93.4 | |
| 3,5-diISA+V | 11.59 ± 0.0606c | 0.0695 ± 0.0003bc | 11.661 ± 0.060c | 1.2 ± 0.01b | 7.99 ± 0.14 | 90.8 | |
*Total I content determinate after TMAH extraction (see Figure 1).**Organic-I—I in all organic I compounds analyzed in roots and leaves of lettuce from hydroponic Experiment No. 1 (see Supplementary Table 4, 5). Sum content of I in form: 5-ISA, 3,5-diISA, I-Tyr, T3-Na, T3, 2-IBeA, 4-IBeA, 2,3,5-triIBeA in relation to the total I content. Means in the column followed by different letters separately for each experiments differ significantly at P < 0.05.