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. 2019 Mar 14;26(1):66–76. doi: 10.5603/CJ.a2018.0009

Table 1.

The gene sequences of shRNA oligo for vector construction and the primers for polymerase chain reaction.

Primers Gene sequences
c-Ski-shRNA1 F1: 5′-CCG GAC GTC TTA CCG TGC CTA TTA CCT CGA GGT AAT AGG CAC GGT AAG ACG TTT TTG-3′
R1: 5′-AAT TCA AAA AAC GTC TTA CCG TGC CTA TTA CCT CGA GGT AAT AGG CAC GGT AAG ACGT-3′
c-Ski-shRNA2 F2: 5′-CCG GGA ATC TGC CAC TCT CAG AAT ACT CGA GTA TTC TGA GAG TGG CAG ATT CTT TTT G-3′
R2: 5′-AAT TCA AAA AGA ATC TGC CAC TCT CAG AAT ACT CGA GTA TTC TGA GAG TGG CAG ATT C-3′
c-Ski-shRNA3 F3: 5′-CCG GCA GTG TCA GCG AGT GAG AAA GCT CGA GCT TTC TCA CTC GCT GAC ACT GTT TTT G-3′
R3: 5′-AAT TCA AAA ACA GTG TCA GCG AGT GAG AAA GCT CGA GCT TTC TCA CTC GCT GAC ACT G-3′
shScramble 5′-CCG GCC TAA GGT TAA GTC GCC CTC GCT CGA GCG AGG GCG ACT TAA CCT TAG GTT TTT G-3′
c-Ski F 5′-CGA GAC AGC TTC TAC TCC TAC A-3′
R 5′-TAG TAA GGG GTT CTG GTG CC-3′
GAPDH F 5′-GGA GAT TGT TGC CAT CAA CG-3′
R 5′-TTG GTG GTG CAG GAT GCA TT-3′