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. Author manuscript; available in PMC: 2021 Apr 30.
Published in final edited form as: Adv Parasitol. 2020 Feb 5;107:25–96. doi: 10.1016/bs.apar.2019.11.001

Table 2.

Inventory of conserved and novel ventral disc and median body proteins.

GiardiaDB Protein name Protein family PFAM BB AX DSC MB FN SN MS Other localization Evidence References
Disc-associated proteins (DAPs)
GL50803_15101 Alpha17-giardin Annexin PF00191 X X HOM, EPI Weiland et al. (2005)
GL50803_7796 Alpha2-giardin Annexin PF00191 X X GFP House et al. (2011)
GL50803_11683 Alpha3-giardin Annexin PF00191 X HOM, EPI Weiland et al. (2005)
GL50803_7797 Alpha5-giardin Annexin PF00191 X X HOM, EPI Weiland et al. (2005)
GL50803_11649 Alpha8-giardin Annexin PF00191 X IFA Wei et al. (2010)
GL50803_17097 Ankyrin repeat protein Ankyrin repeat domain PF12796 X X GFP Nosala et al. (2019)
GL50803_14800 Ankyrin repeat protein Ankyrin repeat domain PF12796 X X Marginal plates GFP Nosala et al. (2019)
GL50803_13590 Ankyrin repeat protein Ankyrin repeat domain PF12796 X X GFP Nosala et al. (2019)
GL50803_137684 Ankyrin repeat protein Ankyrin repeat domain PF12796 X X GFP Nosala et al. (2019)
GL50803_14681 Ankyrin repeat protein Ankyrin repeat domain PF12796 X X GFP Nosala et al. (2019)
GL50803_3760 Ankyrin repeat protein Ankyrin repeat domain PF12796 X Cytoplasm GFP Nosala et al. (2019)
GL50803_10219 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_103807 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_103810 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_112557 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_12139 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_13766 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_14859 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_14872 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_15576 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_16843 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_17053 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_17096 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_17551 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_23492 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_24194 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_40016 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_5188 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_7268 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_7414 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_8850 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_9515 Ankyrin repeat protein Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_5358 Aurora kinase Aurora kinase PF00069 X X X X Nuclei EPI Davids et al. (2008)
GL50803_4812 Beta-giardin SF-assemblin PF06705 X IFA Baker et al. (1988)
GL50803_86676 Delta-giardin SF-assemblin PF06705 X IFA, GFP Nosala et al. (2019)
GL50803_16263 DIP13 DIP13 None X X GFP Nosala et al. (2019)
GL50803_5568 DUF866 domain protein DUF866 PF05907 X X X GFP Nosala et al. (2019)
GL50803_3256 Epsin ENTH PF01417 X EPI Ebneter and Hehl (2014)
GL50803_17563 ERK1 kinase CMGC MAPK PF00069 X X X X EPI Ellis et al. (2003)
GL50803_41512 Flagella associated protein Rib72 DUF1126 PF06565 X X X EPI, GFP Nosala et al. (2019) and Lauwaet et al. (2011)
GL50803_17230 Gamma-giardin None None X IFA, EPI Nohria et al. (1992) and Kim and Park (2019)
GL50803_102455 GiKIN6a Kinesin-6 PF00225 X X GFP Nosala et al. (2019)
GL50803_101326 Hypothetical protein None None X X GFP Nosala et al. (2019)
GL50803_16935 Hypothetical protein None None X X GFP Nosala et al. (2019)
GL50803_24537 Hypothetical protein None None X X GFP Nosala et al. (2019)
GL50803_2556 Hypothetical protein None None X X GFP Nosala et al. (2019)
GL50803_33866 Hypothetical protein None None X X GFP Nosala et al. (2019)
GL50803_6709 Hypothetical protein None None X X GFP Nosala et al. (2019)
GL50803_7520 Hypothetical protein None None X X GFP Nosala et al. (2019)
GL50803_86815 Hypothetical protein None None X X Cytoplasm GFP Nosala et al. (2019)
GL50803_4239 Hypothetical protein None None X Cytoplasm GFP Nosala et al. (2019)
GL50803_6171 Hypothetical protein None None X Cytoplasm GFP Nosala et al. (2019)
GL50803_20688 Hypothetical protein None None X GFP Dawsonlab (GiardiaDB)
GL50803_4852 Hypothetical protein None None X EPI Lauwaet et al. (2011)
GL50803_10181 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_10232 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_10524 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_13651 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_15499 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_15918 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_16342 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_17412 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_3934 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_5883 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_6751 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_8726 Hypothetical protein None None X GFP Nosala et al. (2019)
GL50803_16343 Median body protein None None X GFP Nosala et al. (2019)
GL50803_16424 Mlf1IP domain protein Mlf1IP PF10248 X X X GFP Nosala et al. (2019)
GL50803_13981 Nek kinase GK185 NEK, ankyrin PF00069, PF12796 X GFP Nosala et al. (2019)
GL50803_17231 Nek kinase GK186 NEK, ankyrin PF00069, PF12796 X GFP Nosala et al. (2019)
GL50803_16272 Nek kinase GK187 NEK, ankyrin PF00069, PF12796 X GFP Nosala et al. (2019)
GL50803_10893 Nek kinase GK193 NEK PF00069 X X GFP Nosala et al. (2019)
GL50803_3957 Nek kinase GK212 NEK, ankyrin PF00069, PF12796 X GFP Nosala et al. (2019)
GL50803_11554 Nek kinase GK249 NEK PF00069 X GFP Nosala et al. (2019)
GL50803_16279 Nek kinase GK256 NEK PF00069 X X X HOM, EPI Davids et al. (2011) and Manning et al. (2011)
GL50803_24321 Nek kinase GK261 NEK PF00069 X GFP Nosala et al. (2019)
GL50803_4912 Nek kinase GK265 NEK PF00069 X GFP Nosala et al. (2019)
GL50803_92498 Nek kinase GK270 NEK PF00069 X X EPI Davids et al. (2011) and Manning et al. (2011)
GL50803_5489 Nek kinase GK271 NEK PF00069 X GFP Nosala et al. (2019)
GL50803_4977 Nek kinase GK282 NEK PF00069 X GFP Nosala et al. (2019)
GL50803_11775 Nek kinase GK301 NEK, ankyrin PF00069, PF12796 X X X GFP Nosala et al. (2019)
GL50803_7710 Nek kinase GK445 NEK PF00069 X GFP Dawsonlab (GiardiaDB)
GL50803_4410 SALP-1 SF-assemblin PF06705 X GFP Nosala et al. (2019)
GL50803_17090 SAM domain protein SAM PF00546 X GFP Nosala et al. (2019)
GL50803_5010 Ser/Thr phosphatase PP2A-2 catalytic subunit Calcineurin-like phosphoesterase PF00149 X X X IFA Lauwaet et al. (2007) and Manning et al. (2011)
GL50803_15410 Ser/Thr protein kinase Ankyrin repeat domain PF12796 X GFP Nosala et al. (2019)
GL50803_103164 SHIPPO-repeat family protein SHIPPO-repeat PF07004 X X GFP Nosala et al. (2019)
GL50803_9148 SHIPPO-repeat family protein SHIPPO-repeat PF07004 X X GFP Nosala et al. (2019)
GL50803_5374 Tubulin-specific chaperone B CAP-GLY PF01302 X GFP Nosala et al. (2019)
GL50803_15218 WD-40 repeat protein WD-40 repeat protein PF00400 X X X X IFA, GFP Nosala et al. (2019)
Median body-associated
GL50803_14748 Conserved hypothetical protein None None X Cytoplasm, nuclei GFP Dawsonlab (GiardiaDB)
GL50803_92760 Hypothetical protein None None X X GFP Dawsonlab (GiardiaDB)
GL50803_12224 Hypothetical protein None None X GFP Dawsonlab (GiardiaDB)
GL50803_35670 Zinc finger domain containing protein Zinc finger domain protein PF16543 X Cytoplasm GFP Dawsonlab (GiardiaDB)
Funis-associated
GL50803_4657 Hypothetical protein None None X X X GFP Dawsonlab (GiardiaDB)
GL50803_17266 Hypothetical protein None None X X X GFP Dawsonlab (GiardiaDB)

Disc-associated proteins (DAPs) have been identified by on homology to known cytoskeletal-associated proteins in other organisms (HOM) or by subcellular localization using heterologous antibodies (IFA), epitope-tagging (EPI), or fluorescent tags (GFP, mNG) (Nosala et al., 2018). Over 90 disc-associated or median body-associated proteins have been localized during an ongoing, publicly available, Giardia C-terminal GFP-tagging project for EuPATHDB (Harb and Roos, 2015) conducted in our laboratory (GFP) to the basal bodies (BB), axonemes (AX), disc (DSC), median body (MB), funis (FN), supernumerary MTs (SN) or mitotic spindles (MS).