Fig 4. Functional classification of Differentially Expressed (DE) genes using Gene Ontology (GO) term enrichment.

GO term enrichment was performed using GofuncR on the list of DE genes in cells treated with 1018 and cells at the centre of swarming colonies relative to cells at the edge of swarming colonies. GO terms were considered significant with a P ≤ 0.05 and a multi-test corrected family-wise error rate (FWER) ≤ 0.1. Cells treated with 1018, but not cells at the centre of swarming colonies, cf. swarming edge cells, exhibited functional enrichment in transport of small molecules, heme metabolism and amino acid biosynthesis/metabolism.