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. 2021 Apr 7;17(4):e1009502. doi: 10.1371/journal.ppat.1009502

Fig 2. HeLa cell DNA damage generated by Trypanosoma cruzi affects translational and replicative machinery.

Fig 2

(A) Isolated chromatin from infected HeLa cells (+) and control or not (-) after (A) 1 h and (B) 48 h and 72 h were immunoblotted with anti-DNPH (reveals carbonylated proteins). Anti-histone 3 (H3) was used as a loading control. (C) RNA synthesis was observed by 5-Ethynyl Uridine (EU) incorporation in infected HeLa cells or not with T. cruzi after 1 h of infection. (D) population of infected cells or not (control) were incubated with Bromodeoxyuridine (BrdU) and then stained with anti-BrdU (green). Nuclei were stained with DAPI probe (blue). (E) bars represent the percentage of stained (positive) or not (negative) cells anti-BrdU. Three independent experiments were performed. In the Infected population, it was counted cells without T. cruzi (N.I.), cells with a maximum of 10 parasites (<10), and cells with more than 10 parasites (>10).