Fig 3. mRNA expression and recruitment of DNA repair enzymes from HeLa cells are regulated by Trypanosoma cruzi infection.

(A) Isolated chromatin from infected cells (+) or not (-), 1 h, and 6 h post-infection were immunoblotted with anti-PARP and anti-OGG1. Anti-histone 3 (H3) was used as a loading control. (B) Relative expression (ddCT) from genes PARP1, OGG1 and XRRC1 from infected HeLa cells compared to control 6 h post-infection. ACTB gene was used as the endogenous control. ***p<0.001. (C) Isolated chromatin from infected cells (+) or not, 48 h and 72 h post-infection were immunoblotted with anti-PARP1 and loading control (H3). (D) Immunofluorescence of infected cells after 72 h of infection stained with anti-PARP1 (green) and DAPI probe (blue). Scale bar: 10 μm.