Fig 6. Readthrough-permissive combinations of stop codon and nt +4 identities have additive effects on readthrough efficiency and are avoided in the genome.
A. Heatmap of median readthrough efficiency of genes containing particular combination of stop codon and nt +4 relative to median readthrough efficiency of all genes in the sample. Statistical analysis as in A. B. Heatmap showing residuals of χ2 test of independence between stop codon and nt +4 identities. Significant values (p < 0.05), represented by larger tile size, indicate the significant association between stop codon and nt +4 identities. Positive (red) residuals specify positive association (attraction) and negative (blue) residuals negative association (repulsion) between variables.