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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Mol Microbiol. 2020 Nov 21;115(5):849–859. doi: 10.1111/mmi.14635

Fig. 2.

Fig. 2.

Localization of three proteins with potential roles in signaling to different components of the flagellar membrane in L. mexicana promastigotes. A) The flagellar glucose transporter GT1, tagged at the C-terminus with GFP, localizes along the length of the flagellum (white arrow). B) The putative Ca2+ channel encoded by LmxM.33.0480, fused at its C-terminus to mNeonGreen, is restricted to the flagellum attachment zone (white arrow), a localized adhesion between the flagellar membrane and the flagellar pocket membrane near the base of the flagellum. C) A cNMP-PDE encoded by LmxM.08_29.2440, fused at its C-terminus to mNeonGreen, localizes to the flagellum with a concentration at the distal tip (white arrow) in many parasites. All images are of live parasites imaged in CyGel and represent superpositions of fluorescence and differential interference microscopy. Scale bars are 5 μm.