Skip to main content
. 2021 May 3;131(9):e142088. doi: 10.1172/JCI142088

Figure 3. IgG RNA+ cells in relation to other tissue structures and cell types.

Figure 3

(A) ASCs are found infiltrating tissue distant from vascular structures, as well as adjacent to and within small capillaries. IgG-producing cells identified by FISH in a healing biopsy are found independently in tissue (lower left) as well as proximate to small capillaries (lower right) in biopsy specimens taken during healing after HSV-2 reactivation. Faint green signal is due to collagen autofluorescence. Blood vessel endothelial cells identified by vWF IF (green); IgG RNA+ cells, magenta. Scale bars: 200 μm; insets, 25 μm. vWF and IgG colocalization by combination FISH and IF was performed in specimens from 6 participants. While capillaries were identified in all samples, IgG cells were not present within these vascular structures in all samples. (B) IgG-producing cells by FISH (left) clustered in an area of dense T cell infiltration (right). CD4, green; CD8, red. Shown are serial sections of the same specimen. Scale bars: 50 μm. IgG FISH and T cell IF were performed in serial biopsy specimens from 4 participants. (C) Clustered IgG (red) and CD4 (green) by FISH; absence of vWF staining was confirmed. Scale bar: 50 μm. IgG and CD4 FISH was performed in specimens from 8 participants.