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Immunofluorescence imaging and orthogonal plane views of HUVECs with cell-associated Tie1-Fc (yellow), scale bar = 5 μm. Red bars indicate cross-sectional views in xz and yz images, which were produced by combining z-stack images using FIJI/ImageJ. The majority of Tie1 appears at the apical or basolateral cell surface. Representative images from four individual cells are shown.
