Extended Data Fig. 9. Tie1 mutation does not affect Erk1/2 phosphorylation in vivo.

(a) Representative Western blots and (b) quantification of pErk1/2 from lung tissue harvested from 7-day-old pups, n = 6 per genotype. pErk1/2 values were normalized to the corresponding total Erk1/2 levels and are reported relative to the wild-type animals. Proteins were imaged on the same blot to allow for direct comparison between samples. Data represent mean ± s.e.m., unpaired, two-tailed Student’s t test.