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. Author manuscript; available in PMC: 2021 Apr 30.
Published in final edited form as: Nat Chem Biol. 2020 Oct 5;17(2):178–186. doi: 10.1038/s41589-020-00657-7

Figure 5. Loss of the HS-Tie1 interaction decreases Tie1-Tie2 heterodimerization.

Figure 5.

(a) Representative Western blots and (b) quantification of Tie2 co-immunoprecipitation by Tie1 from HUVECs after treatment with or without heparinase (HSase) and chemical crosslinking. Co-immunoprecipitated Tie2 values were normalized to the corresponding Tie1 levels and are reported relative to the non-HSase-treated control. Data represent mean ± s.e.m., n = 3 biologically independent samples, unpaired, two-tailed Student’s t test. (c) Representative images and (d) quantification of Tie1/Tie2 heterodimers (green) revealed by proximity ligation assay from HUVECs with and without HSase treatment, scale bar = 20 μm. Nuclear staining by DAPI is shown in blue. Data represent mean ± s.e.m., n = 3 biologically independent samples, unpaired, two-tailed Student’s t test.